Journal: Scientific Reports

Article Title: High fidelity fibre-based physiological sensing deep in tissue

doi: 10.1038/s41598-019-44077-7

Figure Lengend Snippet: Optical setup to allow fibre based sensing. A 520 nm laser source (10 µW power and 100 ms exposure) was used as a pump source to excite both the pH and oxygen sensors. The pump illumination was launched from a single mode fibre (SMF) collimated by an aspheric lens (L1), passing through a band pass filter (BP) removing unwanted long wavelength light, and reflected by the dichroic mirror (DM), then focused with an identical aspheric lens (L2) and selectively coupled into the fibre core (with XY control of the sensing fibre mount). The returned light from the fibre core was collimated by L2, passed through the dichroic mirror and a long pass filter (LP, removing any reflected pump light). The fluorescence was then focused by lens L3 into a 50 µm core multimode fibre (MMF) patch cable, and directed to a spectrometer (Ocean Optics QEPro). The triggering unit (TTL pulse generator) controlled both laser and spectrometer allowing on demand short integration time (100 ms) synchronised measurements.

Article Snippet: The fluorescence was then focused by lens L3 into a 50 µm core multimode fibre (MMF) patch cable, and directed to a spectrometer (Ocean Optics QEPro).

Techniques: Fluorescence

Journal: Pharmaceutics

Article Title: Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

doi: 10.3390/pharmaceutics16020252

Figure Lengend Snippet: Schematic experimental steps for ( A ) extraction and isolation of BRA from Arrabidaea brachypoda roots and ( B ) preparation of BRA-loaded F127 micelles by the solid dispersion method.

Article Snippet: The AFM analysis of the F127 and BRA-loaded F127 micelles was performed using a Multimode 8 microscope (Bruker, Santa Barbara, CA, USA). qpBioT (Nanosensor) probes were used with a nominal spring constant of 0.08 N/m.

Techniques: Extraction, Isolation, Dispersion

Journal: Pharmaceutics

Article Title: Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

doi: 10.3390/pharmaceutics16020252

Figure Lengend Snippet: ( A ) Schematic diagram of a typical force versus separation curve obtained with AFM. ( B ) Topographic maps of the nanostructured micelles. ( C ) Root mean square of the roughness plot. The values found for the empty F127 micelles (F127). Significant value differences were determined by ANOVA statistics with post-test Tukey p < 0.05. ( D ) Graph of adhesion forces with mean values of 3.5 ± 0.9 nN and 1.6 ± 0.3 nN, respectively, for F127 and LF-B500. The inner image represents the behavior of the AFM probe during the film interaction in the analysis of adhesion forces. ( E ) Young’s modulus plot with mean values of 12.4 ± 1.4 MPa for the F127 and 12.8 ± 1.0 MPa for the LF-B500. The inner image represents the behavior of the AFM probe during the film interaction in the analysis of elastic forces. (*) Values with a significant difference in the ANOVA statistics with post-test Tukey p < 0.05.

Article Snippet: The AFM analysis of the F127 and BRA-loaded F127 micelles was performed using a Multimode 8 microscope (Bruker, Santa Barbara, CA, USA). qpBioT (Nanosensor) probes were used with a nominal spring constant of 0.08 N/m.

Techniques:

Journal: Pharmaceutics

Article Title: Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

doi: 10.3390/pharmaceutics16020252

Figure Lengend Snippet: ( A ) Picture of the liquid formulation LF-B500 showing physical stability after 72 h of preparation. ( B ) Chemical structure of brachy-dins A, B, and C. ( C ) Representation of the F127 copolymer composition. ( D ) Schematic representation of worm-like F127 micelles observed by morphology analysis.

Article Snippet: The AFM analysis of the F127 and BRA-loaded F127 micelles was performed using a Multimode 8 microscope (Bruker, Santa Barbara, CA, USA). qpBioT (Nanosensor) probes were used with a nominal spring constant of 0.08 N/m.

Techniques: Formulation

Journal: Pharmaceutics

Article Title: Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

doi: 10.3390/pharmaceutics16020252

Figure Lengend Snippet: FTIR frequencies for neat BRA and BRA-loaded F127 micelles (LF-B500).

Article Snippet: The AFM analysis of the F127 and BRA-loaded F127 micelles was performed using a Multimode 8 microscope (Bruker, Santa Barbara, CA, USA). qpBioT (Nanosensor) probes were used with a nominal spring constant of 0.08 N/m.

Techniques:

Journal: Pharmaceutics

Article Title: Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

doi: 10.3390/pharmaceutics16020252

Figure Lengend Snippet: AFM representative images for ( A ) empty F127 micelles and ( B ) BRA-loaded F127 micelles: height ( aI – aIII ), adhesion force maps ( bI – bIII ), and Young’s modulus ( cI – cIII ).

Article Snippet: The AFM analysis of the F127 and BRA-loaded F127 micelles was performed using a Multimode 8 microscope (Bruker, Santa Barbara, CA, USA). qpBioT (Nanosensor) probes were used with a nominal spring constant of 0.08 N/m.

Techniques:

Journal: Pharmaceutics

Article Title: Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

doi: 10.3390/pharmaceutics16020252

Figure Lengend Snippet: Peak (P) ± SD, area (A), hydrodynamic diameter (Dh), polydispersity Index (PDI), and ζ potential of empty and BRA-loaded F127 micelles acquired by the DLS technique in triplicate at 25° C.

Article Snippet: The AFM analysis of the F127 and BRA-loaded F127 micelles was performed using a Multimode 8 microscope (Bruker, Santa Barbara, CA, USA). qpBioT (Nanosensor) probes were used with a nominal spring constant of 0.08 N/m.

Techniques:

Journal: Pharmaceutics

Article Title: Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

doi: 10.3390/pharmaceutics16020252

Figure Lengend Snippet: Differential pulse voltammograms on the GCE were recorded in different conditions: supporting electrolyte only (blank), in the presence of BRA, F127, and BRA-loaded F127 micelles in 0.1 mol L −1 phosphate buffer, pH 5.5, pulse amplitude = 50 mV and v = 25 mV s −1 .

Article Snippet: The AFM analysis of the F127 and BRA-loaded F127 micelles was performed using a Multimode 8 microscope (Bruker, Santa Barbara, CA, USA). qpBioT (Nanosensor) probes were used with a nominal spring constant of 0.08 N/m.

Techniques:

Journal: Pharmaceutics

Article Title: Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

doi: 10.3390/pharmaceutics16020252

Figure Lengend Snippet: In vitro cytotoxicity assay of BRA-loaded F127 micelles against ( A ) RAW 264.7 cell line murine and ( B ) Leishmania amazonensis promastigotes. The assay was performed for different F127 copolymer concentrations ( w / v ): 0.5% (red bars), 0.25% (green bars), and 0.125% (blue bars), scanning a BRA concentration range of 500 to 1.9 µg/mL. The dose–response curves were accessed by linear regression for 0.125% w / v of the F127 copolymer. ( C ) 50% of RAW cell proliferation was achieved for a BRA concentration of 171 µg/mL, and ( D ) 50% of Leishmania amazonensis promastigotes proliferation was achieved for a BRA concentration of 16.06 µg/mL. The results correspond to (means ± SD) of individual samples tested in triplicate. (*) p < 0.05, compared to the positive controls (DMSO and pentamidine).

Article Snippet: The AFM analysis of the F127 and BRA-loaded F127 micelles was performed using a Multimode 8 microscope (Bruker, Santa Barbara, CA, USA). qpBioT (Nanosensor) probes were used with a nominal spring constant of 0.08 N/m.

Techniques: In Vitro, Cytotoxicity Assay, Concentration Assay